Development of an antigen Enzyme-Linked AptaSorbent Assay (ELASA) for the detection of swine influenza virus in field samples

Influenza viruses are highly variable pathogens that infect a wide range of mammalian and avian species. According to the internal conserved proteins (nucleoprotein: NP, matrix proteins: M), these classified into type A, B, C, D. A virus in swine is significant importance industry since it responsible for endemic infections lead high economic loses derived from poor weight gain, reproductive disorders, role plays Porcine Respiratory Disease Complex (PRDC). To date, influenza (SIV) diagnosis continues be based complex expensive technologies such as RT-qPCR. In this study, we aimed improve actual tools by implementation aptamers capture molecules. First, three different have been selected using target recombinant NP expressed insect cells. Then, molecules used development an Enzyme-Linked AptaSorbent Assay (ELASA) combination with specific monoclonal antibodies detection. total 171 field samples (nasal swabs) evaluated newly developed assay obtaining 79.7% 98.1% sensitivity specificity respectively, real time RT-PCR standard assay. These results suggest promising method could detection analysis laboratories facilitating surveillance labours. • Aptamers potential diagnostic methods. good performance was obtained combining antibodies. The ELASA will simplify reduce costs surveillance.